Detection of Escherichia coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Vibrio cholerae, and Campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Specific detection of Salmonella spp. by multiplex polymerase chain reaction.
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria. In addition to the phoP primers, the Hin and the H-1i primers, which tar...
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The aim of the work was to collect, evaluate, summarize and compare heat resistance data reported for Campylobacter, Enterococcus, Escherichia, Listeria, Salmonella and Yersinia spp. The work was limited to resistance in liquids with pH values 6-8. Results obtained under similar experimental conditions were sought. Thermal destruction lines for the various bacterial groups studied were construc...
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Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...
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The antigenic type strains for Yersinia enterocolitica antigens O:1 to O:34 were examined and their antigenic relationships with the type strains of Escherichia coli, Shigella spp., and Salmonella spp. were determined. Y. enterocolitica O:5,27 was antigenically identical to E. coli O97 and Y. enterocolitica O:11 was antigenically identical to E. coli O98.
متن کاملSpecific Detection of Salmonella spp. in Food by Multiplex Polymerase Chain Reaction
A multiplex PCR (mPCR) assay was developed for the detection of multiple Salmonella serotypes in different kind of food products. To increase specificity of this molecular method, three sets of oligonucleotide primer were used to detect the most prevalent salmonella species. PhoP primers specific to the PhoP/PhoQ loci of coliform pathogenic bacteria such as Salmonella, Escherichia coli and citr...
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ژورنال
عنوان ژورنال: Diagnostic Microbiology and Infectious Disease
سال: 2009
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2008.09.006